The Effect of Different Solvents on the Content of Black Cumin Seed Extract (Nigella sativa)

Phytochemical screening GCMS Black Cumin Seeds

Authors

  • Desi Hariutami Clinical/ Medical Laboratory Science Program, Universitas Muhammadiyah Semarang, Semarang, Indonesia, Indonesia
  • Sri Darmawati
    ciciekdarma@unimus.ac.id
    Clinical/ Medical Laboratory Science Program, Universitas Muhammadiyah Semarang, Semarang, Indonesia, Indonesia
  • Atna Permana Faculty of Health, Mohammad Husni Thamrin University, Jakarta, Indonesia, Indonesia
  • Zuraida Zuraida Faculty of Health, Mohammad Husni Thamrin University, Jakarta, Indonesia, Indonesia
December 9, 2023

February 28, 2024

February 29, 2024
February 29, 2024

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Seeds of black cumin (Nigella sativa L.), also called "black cumin," are an herbal plant from the Ranunculaceae family. The objective of this study was to determine the yield of black cumin seed extract using 96% ethanol, N-hexane, and ethyl acetate as solvents, as well as to determine the results of phytochemical screening tests and the active ingredient content using GCMS. The stages of the research included the extraction process, phytochemical screening test, and GCMS. The study's findings on the yield of black cumin seed extract using the three solvents showed that the solvent N-Hexane produced the highest yield, at 28.09 percent. Based on phytochemical screening tests, black cumin seeds were positive for containing secondary metabolites such as alkaloids, saponins, phenolic flavonoids, triterpenoids, steroids, and glycoxides. Other potential compounds vizhexadecenoic acid, ethyl ester, Methyl (8e,11e)-8,11- octadecadienoate, 12-octadecadienoic acid (z,z)-, Grape seed oil.