Optimasi Sintesa Nanokoloid Human Serum Albumin sebagai Agen Limfoskintigrafi Menggunakan Central Composite Design-Response Surface Methodology

The prevalence of cancer in Indonesia is increasing and various cancer treatments still have shortcomings, cancer recurrence still occurs. Lymphoscintigraphy is a method for detecting disorders of the lymphatic system, which are closely related to cancer cells. Human serum albumin nanocolloid as a lymphoscintigraphic agent consists of particles measuring <100 nm and this size is a parameter that must be met. Optimization of the synthesis of HSA nano colloids with the Central Composite Design Response Surface Methodology method was carried out to obtain the appropriate particle size, using the variables of pH, reaction temperature and reaction time. The method used can reduce the time and cost required for synthesis optimization. The optimization results show that the developed method has a significant effect. The recommended optimum conditions (prediction) are synthesis at pH 7, reaction temperature of 70oC and reaction time of 24 minutes. The particle size prediction results from the software were verified against the experimental results in the laboratory and based on ANOVA analysis it was found that the response value between prediction and validation was smaller than 5%. The optimum prediction could be used in the HSA nano colloid synthesis process.