Antioxidant and Inhibition Lipase Enzyme Activity of Centella asiatica Leaf Extract

Antioxidants Asiaticoside Centella asiatica extract In vitro Lipase enzyme

Authors

  • Nanang Yunarto
    nanangyunarto@wdh.ac.id
    Sekolah Tinggi Ilmu Kesehatan Widya Dharma Husada, Tangerang Selatan, Indonesia
  • Ummi Mawadatun STIKes Widya Dharma Husada, Tangerang Selatan, Indonesia
  • Uud Nourma Reswandaru Center of Health Resilience and Resource Policy, Jakarta, Indonesia
  • Ahmad Sopian STIKes Widya Dharma Husada, Tangerang Selatan, Indonesia
  • Ani Isnawati National Research and Innovation Agency of the Republic of Indonesia, Jakarta, Indonesia
  • Sukmayati Alegantina National Research and Innovation Agency of the Republic of Indonesia, Jakarta, Indonesia
July 6, 2023

July 27, 2023

August 31, 2023
August 31, 2023

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Hyperlipidemia and many other metabolic diseases are related to oxidative stress. Centella asiatica is a herbal medicine with reported antioxidant effect in vitro. Centella asiatica contains secondary metabolites asiaticoside which are included in the terpenoid group. The study evaluated the respective antioxidant potential and lipase enzyme inhibition of Centella asiatica leaves extract (CAE). Centella asiatica were extracted in ethanol, and the extract was assayed for the measurement of asiaticoside. Ethanolic extracts of asiaticoside content were prepared for HPLC analysis The antioxidant potential of extracts was assessed by its free radical scavenging activity such as 2, 2-diphenyl -1-picrylhydrazyl as well as reducing. The anti-hyperlipidemic effect was evaluated in vitro lipase inhibitory activity test carried out enzymatically using the ELISA method with simvastatin as a comparison. The results showed asiaticoside contain in CAE 1.26%; the IC50 value of the antioxidant test of CAE was 11.38 g/mL; the IC50 value of the lipase enzyme in the CAE was 26.14 g/mL. The antioxidant activity of CAE is categorized as very strong and has the potential to inhibit lipase enzymes. The study suggests that CAE has the potential to inhibit lipase activity, suppressing lipid digestion and thereby diminishing entry of lipids into the body.